The soluble gst is encoded by eight different genes in humans namely. Western blot of gsttagged fusion protein with antigsttag 00ap at various dilutions. The antigst monoclonal antibody product information is available for printing in the a standard portable document format pdf. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Western blot analysis using monoclonal anti gst antibodies. Anti human gst m11 antisera rabbit anti human gst a11 polyclonal antibody about eagle biosciences, inc. The gst gene fusion system has been introduced into numerous expression vectors.
Validated in wb, ip, elisa, chip, iccif and tested in species independent. The gst tag antibody can be helpful in detecting the fusion protein during purification as well as cleavage of gst from the protein of interest. Download the antigst monoclonal antibody product information 1 page pdf 119 kb. Pdf etrap efficient trapping and purification of target. When polyclonal antibodies are used, problems are sometimes encountered owing to the presence of antie. Graph of elisa measuring levels of bound gsttagged mosquito proteins. This product is prepared by labeling high quality polyclonal goat antigst with our exceptionally bright and photostable cf dyes. Immunogen recombinant gst from schistosoma japonicum expressed in e. Goat anti gst tag hrp polyclonal antibody is developed in goat using a recombinant glutathione stransferase gst from schistosoma japonicum.
This gstfusion protein can then be purified from cells via its high affinity for glutathione. Rabbit polyclonal anti gst epitope tag antibody cited in 17 publications. Etrap efficient trapping and purification of target protein polyclonal antibodies from gstprotein immune sera1 article pdf available in biotechnology and applied biochemistry 574. Cf dyes offer exceptional brightness and photostability.
Compare antigst antibody hrp ab58626 from abcam on. Anti gst tag antibody glutathionestransferase gst is a 26 kd protein that rapidly folds into a stable, highly soluble protein after translation. This gst fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies directed against gst can detect both the fusion protein during purification and the cleavage of gst from the protein of interest. Primary goat antiglutathionestransferase gst reacts with pan iggy antibody selector quickly search hundreds of thousands of antibodies available for purchase from vwr by selecting common antibody features like antigen symbol and name, reactivity, clonality, conjugation, host, and other key factors. C recombinant gst pvx110940tr was used to immunize mice and produce polyclonal antibodies. Tested in immunofluorescence if and flow cytometry flow applications. Blot was incubated in secondary antibody goat anti rabbit igg, hrpconjugated, as09 602, agrisera diluted to 1. Antibody 274577 01 antigst antibody by ge healthcare life sciences. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in tbst at rt with agitation. Pdf difference between monoclonal and polyclonal antibodies. This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification.
Wash three times for 5 min each with 15 ml of tbst. Polyclonal antibodies an overview sciencedirect topics. Mouse igg monoclonal antibody for gst tag detection. Antip53 antibody allowed the sensitive detection of native p53.
This gst fusion protein can then be purified from cells via its high affinity for. Detection of human gst in cytoplasmic fraction by anti gst 3g10 monoclonal antibody ab92 in western blot experiment. Antirat gst ya antiserum antirat gst ya goat polyclonal. Bacterial fusion proteins have been used extensively as antigens for the generation of polyclonal antibodies. Lapin gst polyclonal anticorps pour db, if, ip, ihc, elisa, wb. Glutathione stransferase is used to create the gst gene fusion system. Rozrolimupab is the anti rhd recombinant human polyclonal antibody composed of 25 unique igg1 antibodies and is used for the treatment of immune thrombocytopenia purpura and prevention of isoimmunization in rhnegative pregnant women.
Rabbit anti gsttag polyclonal antibody tcba666 taiclone. Both polyclonal and monoclonal antibodies have been used successfully for isolating genes by means of. Rabbit antihuman gst a11 polyclonal antibody eagle bio. Western blot analysis of schistosoma japonicum purified gst showing detection of 26 kda gst protein using mouse anti gst monoclonal antibody, clone 3e2. Etrap efficient trapping and purification of target protein polyclonal antibodies from gst protein immune sera1 article pdf available in biotechnology and applied biochemistry 574.
Western blot analysis of extracts from transfected cos7 cells overexpressing gstbad and untransfected control, using gst 26h1 mouse mab left or antibad antibody right. A recent version of adobe acrobat reader should suffice to read or print this file. Antigsttag pab polyclonal antibody mbl international. The gst tag antibody mouse genscript offers the gst tag antibody, which is the best antibody in the industry. Immunoaffinity purification of antibodies against gst fusion proteins. Fusion proteins offer an important biological assay for direct proteintoprotein interactions. Western blot analysis of schistosoma japonicum purified gst showing detection of 26 kda gst protein using mouse anti gst monoclonal antibody, clone 3e2 smc549. The main difference between monoclonal and polyclonal antibodies is that. Eagle biosciences has quickly become a leading provider of elisa assay kits, hplc assay kits, molecular biology assays, antibodies, and proteins. Polyclonal antigst is designed to detect glutathiones transferase gst, a common expression tag used in molecular biology. Anti gst may be used in various immunoassays to identify the expression of gst fusion proteins. Background information gst glutathione stransferase is a widely used protein tag encoded by the schistosoma japonicum. Production and evaluation of polyclonal rabbit anti human p53.
Gst tag polyclonal antibody thermo fisher scientific. The use of polyclonal antibodies to screen random peptide phage display libraries often results in the recognition of a large. Gst polyclonal antibody formalinfixed and paraffin embedded human kidney labeled with anti gst polyclonal antibody, unconjugated bs2735r at 1. Western blot analysis of cell and tissue lysates using rabbit anti gstp1 antibody immunohistochemistry of paraffinembedded human liver cancer using rabbit anti gstp1 antibody at a dilution of 1200 immunohistochemistry of paraffinembedded mouse kidney using rabbit anti gstp1 antibody at a dilution of 1200. As29531h488 antigst tag, mouse mab hilyte fluor 488. This polyclonal antibody to human glutathoine stransferase a11 is produced in rabbit and is provided as antiserum. Antiglutathionestransferase gst antibody gst antibody. Glutathionestransferase affinity purified polyclonal rabbit antibody, glutathionestransferase polyclonal antibody, glutathionestransferase polyclonal rabbit antibody.
Anti gst antibodythe best quality antibody in the industry. We do have a number of anti gst antibodies which have been tested and are covered by our abpromise guarantee for use in if. Eurogentec is a pioneer in the in vitro production of monoclonal antibodies. Mouse anti gst antibody, clone vpg66 reacts with glutathionestransferase. Antiglutathionestransf erase gst antibody produced in rabbit is specific for native as well as denaturedreduced forms of glutathionestransferase from schistosoma japonicum. Gst provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Gst tag rabbit antitag, polyclonal, invitrogen 100 g.
Detect gst tag using this antigst tag antibody validated for use in wb. Our enewsletter is a great way to stay up to date on the most recent news and products from cell biolabs. Gst glutathione stransferase is a 26kda protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. The antibody is specific for native as well as denaturedreduced forms of glutathionestransferase from schistosoma japonicum.
Affinity purification of antieg95 and antigst antibodies. Purification of polyclonal anticonformational antibodies for use in. Glutathionestransferase mouse antibody pgstshg, glutathionestransferase mouse antibody 6c7, glutathionestransferase mouse antibody peroxidase conjugated. Anti gst antibody ab9085 rabbit polyclonal gst antibody. This gst fusion protein can then be purified from cells via its high.
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